Histological and ultrastructural analysis of cryopreserved sheep preantral follicles
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چکیده
منابع مشابه
Ultrastructural study of isolated mouse preantral follicles co-cultured with cumulus cells with leukemia inhibitory factor
Background: The ultrastructural analysis of cultured follicles could direct us to understand subcellular changes during in vitro culture. Objective: This study was done to verify the ultrastructural characteristics of in vitro cultured mouse isolated preantral follicles in co-culture system in the presence and absence of leukemia inhibitory factor (LIF) Materials and Methods: Mechanically isola...
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Bovine preantral follicles (40-100 microm diameter at collection) were collected from ovaries of slaughtered cows and cultured in vitro with one of the four treatments: follicle stimulating hormone (FSH; 100 ng/ml) alone; FSH plus epidermal growth factor (EGF; 100 ng/ml); FSH plus insulin-transferrin-selenium (ITS; +1%) or FSH plus hypoxanthine (4 mM) in tissue culture medium (TCM 199) suppleme...
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The aim of the present study was to evaluate the effects of indole-3-acetic acid (IAA) on survival, activation, and growth of caprine preantral follicles using histological and ultrastructural criteria. Pieces of caprine ovarian cortex were cultured for 1 or 5 days in Minimum Essential Medium (MEM control medium) supplemented with different concentrations of IAA (10, 20, 40, or 100 ng/ml). Smal...
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This study was undertaken to examine pre- and postimplantation developmental potency of cryopreserved embryos that had undergone in vitro growth (IVG), maturation (IVM) and fertilization (IVF) of oocytes from the preantral follicle stage. An oocyte culture system for IVG and IVM was used in oocyte-granulosa cell complexes (OGCs) derived from preantral follicles in 12-day-old mice. The rate of o...
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ژورنال
عنوان ژورنال: Animal Reproduction Science
سال: 2006
ISSN: 0378-4320
DOI: 10.1016/j.anireprosci.2005.04.013